Fay Compton

Mary Stuart

Gerald Ames

Bothwell

Ivan Samson

Lord Dudley

John Stuart

George Douglas

Ellen Compton

Queen Elizabeth

Lionel d'Aragon

Moray

Harvey Braban

Ruthven

Irene Rooke

Catherine de Medici

Donald Macardle

Francis II

René Maupré

Rizzio

Ernest A. Douglas

Cardinal

Sydney Seaward

Lord Douglas

Edward Sorley

John Knox

Betty Faire

Mary Beaton

Dorothy Fane

Mary Beaton

Nancy Kenyon

Mary Fleming

Julie Hartley-Milburn

Mary Seaton

Basil Rathbone

Undetermined Subordinate Role

Jack Cardiff